UniAmp Overview

UniAmp (Unique Amplicon) is a computational pipeline to generate PCR primers specific to a target genome.

The UniAmp pipeline can be conceptually split into 4 parts:
1. Build directory of query genomes with high sequence similarity to target genome.
2. Retrieve unique sequences in a target genome compared to query genomes.
3. Select unique target sequence for primer design.
4. Design primers to unique target sequence.

Visual representation: